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In vivo replication of carcinogen-modified rat liver DNA: Increased susceptibility of 06-methylguanine compared to N-7-methylguanine in replicated DNA to S1-nuclease

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
95
Issue
2
Identifiers
DOI: 10.1016/0006-291x(80)90860-8

Abstract

Abstract In order to characterize rat liver DNA replicated in vivo on a carcinogen-damaged template, the replicated DNA was treated with S 1-nuclease and the release of ( 14C)-dimethyl-nitrosamine induced 0 6-methylguanine, a lesion associated with miscoding and N-7-methylguanine, a lesion that does not miscode were monitored. The results indicated that both the methylated guanines became susceptible to S 1-nuclease upon replication. However, a greater percentage of 0 6-methylguanine (22% of the total 0 6-methylguanine present in the DNA) compared to N-7-methylguanine (4% of the total N-7-methylguanine present in the DNA) was rendered acid soluble by S 1-nuclease. The preferential release of 0 6-methylguanine compared to N-7-methylguanine from replicated DNA was interpreted to indicate its occurrence in local denatured regions probably generated as a result of misbase pairing.

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