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Use of chromatographic data to determine the molecular weight of a solute eluted from a liquid chromatographic column

Journal of Chromatography A
Publication Date
DOI: 10.1016/s0021-9673(01)96350-2
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Abstract The diffusivities of 69 different compounds in a n-hexane—ethyl acetate solvent mixture have been determined and a precise relationship between solute diffusivity, molecular weight and molar volume established. The band dispersion for each of the same solutes has also been measured employing a liquid chromatographic column designed to emphasize the resistance-to-mass transfer factor and minimize thermal effects resulting from the use of high pressures and high mobile phase velocities. The effect of the capacity ratio of a solute on the resistance-to-mass transfer factor is determined and the relationship between the bandwidth of a solute, its diffusivity and its molecular weight established. A procedure is outlined for the determination of the molecular weight of a solute from the measurement of its bandwidth, when eluted from a liquid chromatographic column, within an error of 13% for 90% of the solutes examined providing the density of the solute lies between 0.85 and 1.25 g/ml.

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