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Polymerase Chain Reaction-Based Site-Directed Mutagenesis Using Magnetic Beads

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
234
Issue
2
Identifiers
DOI: 10.1006/abio.1996.0074

Abstract

Abstract Thyroglobulin double mutants with various substitutions were obtained with a new polymerase chain reaction-based mutagenesis technique. Maximum length of megaprimer and efficiency of mutagenesis were improved by purification of single-stranded DNA, using the avidin–biotin interaction. This method might allow the construction of large libraries of DNA, mutated at different sites.

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