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Kinetics of phosphorylation and dephosphorylation reactions in the Escherichia coli chemotaxis signal transduction system

Authors
Publisher
McGill University
Publication Date
Keywords
  • Biology
  • Microbiology.
Disciplines
  • Biology

Abstract

We investigated the effect of different factors on the rates of CheY phosphorylation and dephosphorylation, utilizing purified proteins and working at appropriate time scales. Furthermore, we defined the kinetics for phospho-transfer from CheA to CheY and for CheY dephosphorylation under a standard set of conditions. Having established these assays we were now in a position to determine how other components of the chemotaxis system, such as receptors, CheW, and CheZ influence the rate of CheY phosphorylation and dephosphorylation. In addition, we investigated the rates of CheB phosphorylation and dephosphorylation. CheB, the chemotaxis methylesterase, is phosphorylated by CheA in much the same fashion as CheY and therefore has the potential to compete with CheY for phospho-CheA. By investigating the kinetics of CheY and CheB phosphorylation and dephosphorylation, we hope to acquire a better quantitative understanding of the chemotaxis signal transduction system. (Abstract shortened by UMI.)

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