Abstract The effects of high hydrostatic pressure (HHP) on the rheological properties of Aloe vera suspension during storage at 4°C were investigated. Aloe vera suspension was pressurised at 300, 400 and 500MPa for 1, 3 and 5min. Aloe vera suspension exhibited shear-thinning behaviour, and the experimental data fit the Herschel–Bulkley model well (R2>0.99). The samples treated at 300MPa for 1, 3 and 5min and 400MPa for 1 and 3min showed a decrease in their yield stress (σ0) values (p<0.05), while the samples treated at 300MPa for 1, 3 and 5min and 400MPa for 1min showed an increase in the consistency coefficient (K) and a decrease in the flow index (n) values (p<0.05). The Aloe vera suspension exhibited thixotropy and behaved like a weak-gel (G′>G″) during storage at 4°C regardless of the pressure treatment. In conclusion, the HHP treatment does not modify the gel properties and the effect on the rheological properties of Aloe vera suspension is dependent of the pressure–time treatment applied. Industrial relevance Given the many beneficial effects of Aloe Vera gel in the health and food industries, its demand has increased considerably. However, its use is affected by its highly perishable nature. The application of high hydrostatic pressure (HHP) is presented as an innovative technology to maintain the original rheological properties of Aloe vera suspension, leading to an enhancement of its functional attributes.