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Separation and quantitation of perbenzoylated glucocerebroside and galactocerebroside by high-performance liquid chromatography

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
138
Issue
2
Identifiers
DOI: 10.1016/0003-2697(84)90825-x
Keywords
  • High-Performance Liquid Chromatography (Hplc)
  • Glucocerebroside
  • Galactocerebroside
  • Perbenzoylation
  • Pellicular Silica
  • Resolution

Abstract

Abstract Perbenzoylated gluco- and galactocerebrosides were separated and quantitated with a modification of existing HPLC methodology. A linear gradient of 0.9–18.4% dioxane in hexane was used to separate the derivatives on a pellicular silica column heated to 65°C. The elevated temperature was the major modification that permitted the separation. This system was compared to a previously reported silica column/isopropanol in hexane system.

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