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Quantitative measurement of events in the mammalian unfolded protein response

Authors
Journal
Methods
1046-2023
Publisher
Elsevier
Publication Date
Volume
35
Issue
4
Identifiers
DOI: 10.1016/j.ymeth.2004.10.012
Disciplines
  • Biology

Abstract

Abstract The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) triggers the unfolded protein response. IRE1, PERK, ATF6, BiP, EDEM, lipid-linked oligosaccharides (LLOs), and XBP1 directly or indirectly participate in this process. This article provides methods used in our laboratory to quantitatively measure the accumulation of mRNAs encoding BiP and EDEM; splicing of XBP-1; cleavage of ATF6; inhibition of protein synthesis by PERK; and extension of LLOs under control and stress conditions.

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