Abstract Bovine serum has been found suitable for preparation of serum pools for evaluation, standardization and quality control programs for cholesterol and triglyceride. Although bovine serum is low in cholesterol and triglyceride, these constituents can be raised in the serum to the desired level by the use of Triton WR-1339, a non-ionic detergent. This substance has been shown previously to cause hyperlipemia in rabbits, rats and dogs. I.v. injection of Triton at the level of 31 mg/kg body weight twice a week produces a sustained hyperlipemia both in steers and in cows. There was no interference from Triton in vivo or in vitro in the determination of cholesterol or triglycéride or the non-lipid constituents of serum tested.