Background: Health promoting lifestyle (HPL) focuses on life promotion through lifestyle which consists of six aspects of “physical activity”, “nutrition”, “health responsibility”, “spiritual growth”, “interpersonal relations” and “stress management”. This lifestyle promotes health and welfare and induces satisfaction, self-persuasion and self-improvement. Considering the importance of the way a new behavior affects “life quality” as a motivational factor for starting and continuing that behavior, this study aimed to determine the relationship between health-promoting lifestyle and its aspects. Materials and Methods: This cross-sectional study was performed on undergraduate students at School of Health, Isfahan University of Medical Sciences, using a census method. Health promoting lifestyle was measured by Health Promotion Lifestyle Profile two and life quality was assessed by the Persian version of QLQ-C30 questionnaire. Data analysis was conducted using descriptive and inferential statistical tests in SPSS. Results: Mean age of the participants was 21.12 years old. From among six aspects of health promoting behaviors, spiritual growth and responsibility with the means of 22.01 ± 2.224 and 20 ± 2.31 had the highest and physical activity with the mean of 17.58 ± 2.9 had the lowest scores, respectively. General life quality of 40.7% students was good and only 19.8% of them had an average global life quality. The highest and lowest frequencies of health-related life quality belonged to very good (58.6%) and excellent (9%) health, respectively. Except for stress management (P = 0.05) and gender of the students, there were no significant relationships between other HPL aspects and gender. There was no statistically significant relationship between global life quality of students and nutrition, physical activity, self-health responsibility and stress management while there was a significant relationship between global life quality and spiritual growth of the students. Health-related life quality and stress management were significantly related to each other; however, no statistically significant relationship was observed between health-related life quality and nutrition, physical activity, interpersonal relations and spiritual growth. Conclusion: There is a significant relationship between adopting health promoting lifestyle and aspects of spiritual growth and stress management on the one hand and general quality of life on the other, at least among students.