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A method for the determination of plasma guanase on finger-tip blood

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
29
Issue
3
Identifiers
DOI: 10.1016/0009-8981(70)90006-9
Disciplines
  • Biology

Abstract

Abstract A method for the determination of guanase in plasma, blood or tissue extracts is described based on the measurement of the radioactive uric acid produced upon the incubation of the sample with radioactive guanine and excess xanthine oxidase. The method is about one thousand times more sensitive than the spectrophotometric methods being capable of measuring 10 −6 IU of the enzyme. Using this method plasma of healthy persons was found to contain about 0.36 IU per liter; red and white blood cells contained undetectable amounts. It was successfully applied to measure guanase activity in blood collected by finger puncture and plasma separated from it.

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