Affordable Access

Fluorescent in situ Hybridisation to Amphioxus Chromosomes

Zoological Society of Japan
Publication Date
  • Biology


Abstract We describe an efficient protocol for mapping genes and other DNA sequences to amphioxus chromosomes using fluorescent in situ hybridisation. We apply this method to identify the number and location of ribosomal DNA gene clusters and telomere sequences in metaphase spreads of Branchiostoma floridae. We also describe how the locations of two single copy genes can be mapped relative to each other, and demonstrate this by mapping an amphioxus Pax gene relative to a homologue of the Notch gene. These methods have great potential for performing comparative genomics between amphioxus and vertebrates.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Advances in fluorescent in situ hybridisation.

on Current Opinion in Biotechnolo... February 1998

Current status of fluorescent in-situ hybridisatio...

on Medical device technology May 2004
More articles like this..