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Peripheral nerve microenvironment: Collection of endoneurially enriched fluid

Authors
Journal
Experimental Neurology
0014-4886
Publisher
Elsevier
Publication Date
Volume
77
Issue
1
Identifiers
DOI: 10.1016/0014-4886(82)90154-6
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Abstract We report the first successful collection and biochemical analysis of endoneurially enriched fluid (EEF) from sciatic nerve of control and lead-intoxicated rats using an implanted polyethylene matrix capsule and collector. The EEF protein concentration was approximately one-half that of rat serum. Protein fractionation by sodium dodecyl sulfate-pore gradient electrophoresis revealed a different protein composition for the endoneurium, EEF, and serum from the same animal. In EEF there is a complex array of protein bands with a molecular weight range of 10 4 to 2 × 10 5 of which albumin was a major constituent. Preliminary results on lead-intoxicated rats suggest that total endoneurial fluid protein may be increased during lead intoxication.

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