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Isolation of a glycoprotein E-deleted bovine herpesvirus type 1 strain in the field

Authors
Publication Date
Keywords
  • Life Sciences :: Veterinary Medicine & Animal Health [F13]
  • Sciences Du Vivant :: Médecine Vétérinaire & Santé Animale [F13]
Disciplines
  • Biology

Abstract

During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal secretions of two cows, eight months after vaccination with a gE-negative live-attenuated vaccine, initially given intranasally, then intramuscularly. The strain isolated was characterised using immunofluorescence, restriction analysis and PCR. All the techniques used identified the isolated virus as a gE-negative BHV-1 phenotypically and genotypically identical to the Za strain used as a control.

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