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RNA interference-induced reduction in CD98 expression suppresses cell fusion during syncytialization of human placental BeWo cells

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
577
Issue
3
Identifiers
DOI: 10.1016/j.febslet.2004.10.047
Keywords
  • Trophoblast
  • Cell Fusion
  • Syncytialization
  • Cd98
  • Rna Interference
Disciplines
  • Biology

Abstract

Abstract The physiological importance of CD98 surface antigen in regulating placental trophoblast cell fusion has been studied in a cell model of syncytialization (the cytotrophoblast cell line BeWo following increased intracellular cAMP by forskolin treatment) using RNA interference. CD98 protein abundance (determined by Western blot) was decreased by 40–50% following double-stranded small interfering RNA transfection. Cell fusion (determined by quantitative flow cytometry) was similarly inhibited and human chorionic gonadotropin secretion was suppressed. These findings show that CD98 is involved in the process of cell fusion necessary for syncytiotrophoblast formation.

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