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In vitro protein translocation into inverted membrane vesicles prepared fromVibrio alginolyticusis stimulated by the electrochemical potential of Na+in the presence ofEscherichia coliSecA

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
264
Issue
1
Identifiers
DOI: 10.1016/0014-5793(90)80751-4
Keywords
  • Seca Protein
  • Protein Secretion
  • Na+Pump
  • Escherichia Coli
  • Vibrio Alginolyticus
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract A protein translocation system was reconstituted from inverted membrane vesicles prepared from Na + pump-possessing Vibrio alginolyticus and purified Escherichia coli SecA. The translocation required ATP and was stimulated by the functioning of the Na + pump, suggesting that the electrochemical potential of Na + but not that of H +, is important for protein translocation in Vibrio.

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