Affordable Access

Enhanced Dupuytren's disease fibroblast populated collagen lattice contraction is independent of endogenous active TGF-β2

Authors
Publisher
BioMed Central
Publication Date
Source
PMC
Keywords
  • Research Article
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

1471-2474-5-41.fm ral ss BioMed CentBMC Musculoskeletal Disorders Open AcceResearch article Enhanced Dupuytren's disease fibroblast populated collagen lattice contraction is independent of endogenous active TGF-β2 Raymond Tse1,4, Jeffrey Howard1,2,4, Yan Wu1,4 and Bing Siang Gan*1,2,3,4 Address: 1Department of Surgery, The University of Western Ontario, London, Ontario, Canada, 2Department of Biochemistry, The University of Western Ontario, London, Ontario, Canada, 3Department of Physiology and Pharmacology, The University of Western Ontario, London, Ontario, Canada and 4Cell & Molecular Biology Laboratory, Hand and Upper Limb Centre, Lawson Health Research Institute, St. Joseph's Health Centre, London, Ontario, Canada Email: Raymond Tse - [email protected]; Jeffrey Howard - [email protected]; Yan Wu - [email protected]; Bing Siang Gan* - [email protected] * Corresponding author Abstract Background: Dupuytren's disease (DD) is a debilitating fibro-proliferative disorder of the hand characterized by the appearance of fibrotic lesions (nodules and cords) leading to flexion contractures of the fingers and loss of hand function. Although the molecular mechanism of DD is unknown, it has been suggested that transforming growth factor-β2 (TGF-β2) may play an important role in the underlying patho-physiology of the disease. The purpose of this study was to further explore this hypothesis by examining the effects of TGF-β2 on primary cell cultures derived from patient-matched disease and normal palmar fascia tissue using a three-dimensional collagen contraction assay. Methods: Fibroblast-populated collagen lattice (FPCL) contraction assays using primary cell cultures derived from diseased and control fascia of the same DD patients were studied in response to exogenous TGF-β2 and neutralizing anti-TGF-β2 antibodies. Results: Contraction of the FPCLs occurred significantly faster and to a greater extent in disease cells compared to control cells. The addi

There are no comments yet on this publication. Be the first to share your thoughts.