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A rapid DNA assembling strategy mediated by direct full-length polymerase chain reaction

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
523
Issue
2
Identifiers
DOI: 10.1016/j.gene.2013.03.140
Keywords
  • Class-Iis Endonuclease
  • Direct Full-Length Polymerase Chain Reaction (Pcr)
  • Dna Assembling
  • Ligation
  • Multiple Site-Directed Mutagenesis

Abstract

Abstract An efficient DNA assembling strategy was developed here modified from Class-IIS endonuclease mediated DNA splicing by directed ligation (SDL). Benefited from the full-length PCR directly using ligation products as template, this strategy required less effort and less time to obtain the assembled full-length DNA. The advantages of this strategy made it a rapid and easy-to-perform gene splicing and multiple site-directed mutagenesis approach especially practicable when more fragments need to be assembled at the same time.

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