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Murine model of primary intraocular lymphoma

American Journal of Ophthalmology
DOI: 10.1016/j.ajo.2005.03.010
  • Biology
  • Chemistry
  • Medicine


Primary intraocular lymphoma (PIOL) is a subtype of central nervous system lymphoma. The only currently available animal model of PIOL is a murine model with intraperitoneal inoculation of Rev-2-T-6 lymphoma cells in newborn syngeneic mice. The authors now report a new experimental model for PIOL. Rev-2-T-6 cells were inoculated into the vitreous of adult BALB/c mice. Mice were funduscopically examined every other day. The mice were sacrificed and the eyes were studied by histology and immunohistochemistry on weeks 3, 5, 6, 7, or 8 after inoculation. Cytokine mRNA experession (Il-2, -4, -6, -10, and IFN-γ and CC chemokine receptor-1 (CCR1) was assayed in the lymphoma cells by microdissection and RT-PCR. IL-10 and -6 levels in the vitreous were measured by ELISA. Within 2 to 4 weeks, tumor cells from the vitreous migrate through the retina had gathered between the RPE and retina. Rarely, Rev-2-T-6 cells may invade through the RPE into the choroid and sclera. Lymphoma cell location was confirmed by immunohistochemistry. The intraocular lymphoma cells produce high levels of IL-10, IFN-γ, and CCR1 transcripts. A high level of IL-10 was detected in the vitreous inoculated with Rev-2-7-6 lymphoma cells. This data suggests that RPE cells constitute a barrier of spread of intraocular lymphoma. Intravitreal inoculation of or Rev-2-T-6 lymphoma cells is a novel model of PIOL in immune competent mice and will aide in understanding the molecular mechanisms of the disease. —Hans E. Grossniklaus

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