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cDNA clones encoding leucine-zipper proteins which interact with G-CSF gene promoter element 1-binding protein

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
299
Issue
1
Identifiers
DOI: 10.1016/0014-5793(92)80094-w
Keywords
  • Granulocyte Colony-Stimulating Factor
  • Transcription Factor
  • Leucine Zipper
  • Protein-Protein Interaction
  • Lipopolysaccharide
Disciplines
  • Biology

Abstract

Abstract The gene expression of granulocyte colony-stimulating factor (G-CSF) is induced by lipopolysaccharide (LPS). GPE1, a cis-controlling element of the G-CSF gene, functions as an LPS-responsive element. GPE1-binding protein (GPE1-BP), a leucine-zipper protein, did not independently activate G-CSF gene expression. Protein blot analysis with biotinylated GPE1-BP revealed that there were nuclear proteins that interact specifically with GPE1-BP. Three leucine-zipper proteins were isolated from mouse cDNA expression libraries by this method: NF-IL6, ATF4, and a novel ATF4-related ATFx. The interactions of these proteins with GPE1-BP may play key roles in G-CSF gene expression.

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