Affordable Access

Publisher Website

Online monitoring of BALB/3T3 metabolism and adhesion with multiparametric chip-based system

Analytical Biochemistry
Publication Date
DOI: 10.1016/j.ab.2007.07.014
  • Silicon Sensors
  • Online Monitoring
  • Cytotoxicity
  • Balb/3T3 Cell Line
  • Cell Adhesion
  • Cell Metabolism
  • Label Free
  • Chemistry


Abstract A multiparametric chip-based system was employed to measure cell adhesion, metabolism, and response to metal compounds previously classified as cytotoxic in immortalized mouse fibroblasts (BALB/3T3 cell line). The system measures in parallel, online, and in label-free conditions the extracellular acidification rates (with pH-sensitive field effect transistors [ISFETs]), the cellular oxygen consumption (with amperometric electrode structures [Clark-type sensors]), and cell adhesion (with impedimetric interdigitated electrode structures [IDESs]). The experimental protocol was optimized to monitor metabolism and adhesion of the BALB/3T3 cell line. A total of 70,000 cells and a bicarbonate buffer-free running low-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal clone serum III and 1 mM Hepes were selected to maintain cells in good conditions on the chip during the measurements performed under perfusion conditions. Cells were exposed to sodium arsenite, cadmium chloride, and cis-platinum at concentrations ranging from 1 to 100 μM. The kinetics of cell response to these compounds was analyzed and suggests that the Clark-type sensors can be more sensitive than IDESs and ISFETs in detecting the presence of high chemical concentration when short exposure times (i.e., 2 h) are considered. The cytotoxicity data obtained from the online measurements of acidification, respiration, and adhesion at 24 h compare well, in terms of half-inhibition concentration values (IC 50), with the ones obtained using 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) test and colony-forming efficiency (CFE) assay. The results show a good sensitivity of the system combined with the advantages of the online and label-free detection methods that allow following cell status before, during, and after the treatment in the same experiment.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Online monitoring of BALB/3T3 metabolism and adhes...

on Analytical Biochemistry Dec 01, 2007


on Tanpakushitsu kakusan koso. Pr... May 1988

Cell-to-substrate contacts in an adhesion-defectiv...

on Journal of Cell Science December 1981
More articles like this..