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A new technique for monitoring hyphal growth of vesicular-arbuscular mycorrhizal fungi through soil

Authors
Journal
Transactions of the British Mycological Society
0007-1536
Publisher
Elsevier
Publication Date
Volume
89
Issue
4
Identifiers
DOI: 10.1016/s0007-1536(87)80075-x
Disciplines
  • Design

Abstract

A system has been designed to monitor indirectly the spread of vesicular-arbuscular mycorrhizal fungal hyphae by periodically examining colonization of ‘trap’ or ‘receiver’ plants separated from inoculated ‘spreader’ plants by a root-free soil region. This system was used to monitor fungal hyphae spread from pre-inoculated clover plants through a 2 cm section containing substrates of various pH values, organic matter contents, nutrient availabilities, and water holding capacities into a section containing non-inoculated clover plants. After 5 weeks, fungal hyphae had colonized receiver plant roots through three of the six substrates. After 10 weeks receiver plant roots were colonized through all substrates but calcined clay. The system functioned well with no cross-contamination and is easily modified for studying other aspects of fungal hyphae growth.

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