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Separation of gliadin at pH 3.1 in a polyacrylamide gel suitable for blotting procedures

Authors
Journal
Journal of Biochemical and Biophysical Methods
0165-022X
Publisher
Elsevier
Publication Date
Volume
10
Identifiers
DOI: 10.1016/0165-022x(85)90064-8
Keywords
  • Gliadin
  • Gel Electrophoresis
  • Blotting
  • Coeliac Disease

Abstract

Abstract A polyacrylamide gel electrophoresis system for the separation of gliadin at acidic pH is described. The gel is cast at neutral pH with polymerization in 20 min. Equilibrium of the gel to pH 3.1 takes place during the electrophoresis. The gel is highly uniform with good mechanical properties and therefore suitable for blotting procedures.

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