Affordable Access

Publisher Website

Detection of IgM antibodies againstBabesia bovisin cattle

Authors
Journal
Veterinary Parasitology
0304-4017
Publisher
Elsevier
Publication Date
Volume
82
Issue
1
Identifiers
DOI: 10.1016/s0304-4017(98)00254-4
Keywords
  • Babesia Bovis
  • Elisa
  • Igm
Disciplines
  • Design
  • Medicine

Abstract

Abstract The diagnosis of acute babesiosis by direct examination of blood smears has some limitations and the indirect serological methods currently in use are designed for detection of IgG, which may not be detectable at an early stage of infection. There is a need, therefore, for rapid and reliable procedures to diagnose acute infections. An ELISA system using a crude antigenic preparation of Babesia bovis was standardized for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Serum samples of cattle imported from tick-free areas collected before and during an immunization process were used to validate the tests. The specificity was 94% and sensitivity 100%. Specific IgM antibodies against B. bovis first appeared on the 11th day post-inoculation (p.i.) in animals infested with Boophilus microplus ticks and on the 19th day p.i. in animals which had been inoculated with infected blood. Antibody titers decreased after Day 33; however, all animals remained positive until the end of the experiment (124 days).

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

An enzyme-linked immunosorbent assay (ELISA) for t...

on Veterinary Parasitology Jan 01, 1997

Evaluation of an ELISA for detection of antibodies...

on Preventive Veterinary Medicine Jan 01, 1998

Detection of IgM antibodies against Babesia bovis...

on Veterinary Parasitology Mar 22, 1999

A fluorescence polarization assay for the detectio...

on Veterinary Microbiology Jan 01, 2002
More articles like this..