Affordable Access

Publisher Website

Molecular Identification and Differentiation ofStaphylococcusSpecies and Strains of Cheese Origin

Authors
Journal
Systematic and Applied Microbiology
0723-2020
Publisher
Elsevier
Publication Date
Volume
27
Issue
2
Identifiers
DOI: 10.1078/072320204322881835
Keywords
  • Amplified Ribosomal Dna Restriction Analysis (Ardra)
  • Pulsed Field Gel Electrophoresis (Pfge)
  • Staphylococcus
  • Cheese Surface
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Summary Amplified Ribosomal-DNA Restriction Analysis (ARDRA) was used to differentiate among 12 species and 4 subspecies of the genus Staphylococcus. With a universal primer pair a 2.4 kbp PCR-product was amplified, including the 16S rDNA, the 16S-23S rDNA interspacer region, and about 500 bp of the 23S rDNA. Species-specific restriction patterns were found using the restriction enzymes Hin dIII and Xmn I separately. Cheese related staphylococci were clearly differentiated. ARDRA results were in good agreement with results of partial sequencing of the 16S rDNA. ARDRA could fully replace the biochemical identification with ID32 Staph (BioMerieux) which was less reliable when staphylococci of cheese origin were analysed. Genomic restriction digests of cheese-related S. equorum strains by Sma I and Sac I gave unique strain-specific restriction patterns which can be used to identify starter staphylococci in a complex microbial environment such as the surface of Red-Smear cheeses.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Molecular identification and differentiation of St...

on Systematic and Applied Microbi... March 2004

Molecular identification and differentiation of Br...

on Systematic and Applied Microbi... January 2007

Molecular identification and differentiation ofBre...

on Systematic and Applied Microbi... Jan 01, 2007

Caseinolysis in cheese by Enterobacteriaceae strai...

on Letters in applied microbiolog... 2003
More articles like this..