Objectives: The purpose of this study was to determine the presence of physical, social, financial and marital stress on mothers of diabetic children and the possible relationship of this stress to the epidemiological characteristics of the mothers. Method: It was a case-control study in which 125 mothers of diabetic children were selected randomly from 10 general and private hospitals in Riyadh city. A control companion group of 110 mothers who were free of chronic illness, was drawn from primary health care centers in the same city. Mothers in both groups were interviewed using a structured questionnaire. Results: In the study group, 110 mothers were interviewed. Physical, social and financial stress was more common in mothers of diabetic children than in mothers in the control group. With the exception of marital stress, the differences were statistically significant. Physical stress was correlated positively with the frequency of blood tests and the working status of mothers. Social stress was correlated positively with the frequency of blood tests; but negatively with child's knowledge of diabetes mellitus. Financial stress was correlated positively with the presence of chronic illness but negatively with family income. Conclusion: Mothers of diabetic children are at risk of being under various kinds of stress. Certain epidemiological factors are associated with the development of the stress. These factors have to be taken into consideration in the management of the disease.