Affordable Access

Publisher Website

Isolation ofBurkholderia cepaciaComplex Genomovars from Waters

Authors
Journal
Systematic and Applied Microbiology
0723-2020
Publisher
Elsevier
Publication Date
Volume
26
Issue
4
Identifiers
DOI: 10.1078/072320203770865909
Keywords
  • Burkholderia Cepaciacomplex
  • Genomovar
  • Water
  • Isolation
  • B. Cepacia
  • B. Multivorans
  • B. Cenocepacia
  • B. Vietnamiensis
  • B. Anthina
Disciplines
  • Medicine

Abstract

Summary The aim of this study was to develop a selective enrichment broth as an aid for the isolation of Burkholderia cepacia complex (Bcc) bacteria from water. To allow growth of all nine genomovars, mixtures of two carbon sources had to be used, i.e. L-arabinose/D-cellobiose or L-arabinose/L-threonine. Selectivity was provided by polymyxin B and 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (C-390). Following enrichment, Bcc bacteria were isolated on a diagnostic O/F agar supplemented with gentamicin. A preliminary bio-diversity study on 28 surface waters yielded five different genomovars, i.e. B. cepacia(genomovar I), B. multivorans, B. cenocepacia, B. vietnamiensis and B. anthina. Drinking waters did not contain Bcc bacteria. However, the genomovar pattern from a given sample varied with the enrichment broth used.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Isolation of Burkholderia cepacia complex genomova...

on Systematic and Applied Microbi... November 2003

Rapid identification ofBurkholderia cepaciacomplex...

on Journal of Microbiological Met... Feb 15, 2013

Genomovar status, virulence markers and genotyping...

on Journal of Cystic Fibrosis Jan 01, 2008
More articles like this..