Affordable Access

Menstrual-cycle-dependent expression of keratan sulphate in human endometrium.

Authors
Publication Date
Disciplines
  • Biology
  • Chemistry
  • Ecology

Abstract

Biochem. J. (1990) 266, 757-763 (Printed in Great Britain) Menstrual-cycle-dependent expression of keratan sulphate in human endometrium Margaret E. HOADLEY, Mourad W. SEIF and John D. APLIN* Departments of Obstetrics and Gynaecology, Biochemistry and Molecular Biology, University of Manchester, Manchester, U.K. Immunochemical methods have been used to detect and characterize two classes of polypeptide-associated keratan sulphate (KS) in epithelial secretions from human endometrium. Monoclonal antibody D9Bl binds to a hormonally regulated sialylated epitope associated with KS in a high relative molecular mass (250000-350000) component that bands as a doublet in SDS/PAGE. These KS chain(s) are sensitive to keratanase, endo-fl-galactosidase and N-glycanase. A second, more highly sulphated, type of KS is also present, that is resistant to all three enzymes. This can be detected using monoclonal antibody 5D4. It is present throughout the menstrual cycle and is associated principally with a component of Mr 140000. Thus secretory KS contributes to the environment of the implanting embryo, may be used as a molecular index of endometrial function and could be important in the establishment of pregnancy. INTRODUCTION During the menstrual cycle, the uterine lining (endo- metrium) undergoes profound structural changes under the influence of ovarian hormones [1,2]. Following men- strual shedding, a proliferative phase returns the tissue to its functional thickness. After ovulation, a secretory phase ensues, in which differentiative changes prepare the endometrium to accept an implanting embryo. The secretory material released by endometrial glandular epithelial cells may play a role in creating a suitable milieu for the blastocyst [2,3]. Recent data also suggest that endometrial secretions may exert paracrine influences on the post-implantation embryo [2-6]. Thus it is important to characterize these secretions and to examine their hormonal dependence and their timing in relation to implantation. We have

There are no comments yet on this publication. Be the first to share your thoughts.