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The effect of pH on α-methyl-d-glucoside accumulation by rat kidney cortex slices

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
0005-2736
Publisher
Elsevier
Publication Date
Volume
345
Issue
2
Identifiers
DOI: 10.1016/0005-2736(74)90255-7

Abstract

Abstract The kinetics of α-methyl- d-glucoside accumulation by rat kidney cortex slices under conditions of varying extracellular pH are compared with values obtained at pH 7.4. At pH below 7.4 there is a diminished initial uptake and reduced influx of the sugar which results in a decrease in the steady-state intracellular pool. This was associated with a decrease in the V of the entry process without affecting the apparent K m of transport. At pH 8 there is no change in the rate of glucoside entry. The efflux of the glucoside, however, is impaired and the steady-state concentration gradient becomes greater than that observed at pH 7.4.

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