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Ozone induction and purification of spruce cinnamyl alcohol dehydrogenase

Authors
Journal
Phytochemistry
0031-9422
Publisher
Elsevier
Publication Date
Volume
32
Issue
3
Identifiers
DOI: 10.1016/s0031-9422(00)95136-7
Keywords
  • Picea Abies
  • Pinaceae
  • Spruce
  • Cinnamyl Alcohol Dehydrogenase
  • Lignin Biosynthesis
  • Ozone.
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Abstract Spruce needle cinnamyl alcohol dehydrogenase (CAD) was induced in seedlings and four-year-old trees two-to-four-fold by ozone concentrations that did not lead to visible damage. CAD thus acted as an early biochemical ozone marker. A highly purified CAD preparation consisting of two ∼42 000 M r subunits was prepared from spruce cell cultures. A polyclonal antibody was strongly inhibitory. The enzyme had high specificity for coniferyl alcohol and NADP +, and was also detected in spruce seedlings and spruce cambial sap.

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