Affordable Access

Publisher Website

Alternative Form of the Dicluster Ferredoxin from the Thermoacidophilic Archaeon,SulfolobusSp. Strain 7

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
206
Issue
2
Identifiers
DOI: 10.1006/bbrc.1995.1080
Disciplines
  • Archaeology

Abstract

Abstract An alternative form of a 7Fe dicluster-type ferredoxin (Fd-B) with a different charge density was purified as a minor component from the aerobic and thermoacidophilic archaeon, Sulfolobus sp. strain 7. Comparison of its properties with those of native 7Fe ferredoxin (Fd-A), a major ferredoxin, was made in terms of the molecular properties, the absorption, circular dichroism and electron paramagnetic resonance spectral properties, and the reactivity coupled with the cognate 2-oxoacid:ferredoxin oxidoreductase at 50°C. Our results suggest that the Sulfolobus 7Fe ferredoxin has two spectroscopically distinct forms with slightly different conformations.

There are no comments yet on this publication. Be the first to share your thoughts.