Affordable Access

Publisher Website

Correction of distortion of histologic sections of arteries

Authors
Journal
Journal of Biomechanics
0021-9290
Publisher
Elsevier
Publication Date
Volume
40
Issue
2
Identifiers
DOI: 10.1016/j.jbiomech.2005.12.019
Keywords
  • Coronary Artery
  • Histology
  • Section Distortion
  • Three-Dimensional Reconstruction
  • Finite Element Model
Disciplines
  • Mathematics
  • Medicine

Abstract

Abstract Histologic sections of arteries can be used to generate three-dimensional (3D) geometric models and identify structural constituents. However, geometric distortions are introduced by fixation, embedding and sectioning; distortions which can, for example, lead to errors in stresses predicted by finite element models. We developed a method to measure and correct for distortions caused by acrylic processing and applied it to intact, healthy porcine coronary arteries. Micro-computed tomography was used to image arteries in the fresh and embedded states. Tissue blocks were sectioned, stained and imaged using a light microscope. Each section contained four registration marks used to determine strains introduced by sectioning and staining. Using these three image sets, 3D geometric models were generated and distortions were measured. Fixation, processing, and embedding resulted in shrinkage of 6.4±2.3% axially and 35.4±5.0% in mean cross-sectional area ( n = 5 ). Shrinkage in a cross section was well characterized by a uniform, equibiaxial strain. Sectioning and staining resulted in additional compressive strains in the sectioning direction of 0.067±0.011 and, in the direction perpendicular to sectioning, of 0.023±0.005 ( n = 5 ). These strains are assumed uniform and form the basis for correcting section geometry. Reconstructions using corrections for sectioning and shrinkage-related distortions had errors of 1.6±0.5% ( n = 5 ) and 4.0±1.7% ( n = 5 ), respectively.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

An unbiased correction factor for cell counts in h...

on Journal of Neuroscience Method... August 1993

An unbiased correction factor for cell counts in h...

on Journal of Neuroscience Method... Jan 01, 1993
More articles like this..