Affordable Access

Live birth after anonymous donation of twice-cryopreserved embryos that had been stored in liquid nitrogen for a cumulative storage time of approximately 13.5 years

Fertility and Sterility
DOI: 10.1016/j.fertnstert.2010.04.071
  • Embryo Donation
  • Re-Cryopreservation
  • Embryo Storage
  • Liquid Nitrogen


Objective To report a live birth after transfer of anonymously donated, twice-cryopreserved embryos that had been stored in liquid nitrogen for approximately 13.5 years. Design Case report. Setting A private assisted reproduction center. Patient(s) A 44-year-old recipient of donated cryopreserved embryos. Intervention(s) Anonymous donation of cryopreserved blastocysts for procreation. Main Outcome Measure(s) Live birth after thawing and replacement of re-cryopreserved blastocysts. Result(s) Fourteen pronuclear-stage embryos and four cleavage-stage embryos were cryopreserved during a primary IVF cycle. In two separate cycles, one cycle for the primary patient and a subsequent cycle for the first embryo donor recipient, the 18 embryos were thawed and grown to the blastocyst stage for transfer. Supernumerary blastocysts (n = 5) not replaced at either of these two thaw cycles were re-cryopreserved and subsequently donated to another embryo donor recipient. Five blastocysts survived the thaw and three were transferred, resulting in a live birth. The embryos were cryopreserved for a cumulative storage time of approximately 4,909 days (13.4 years). Conclusion(s) The longevity (viability) of cryopreserved embryos maintained in liquid nitrogen remains to be determined; cryopreserved embryo donation for procreation should not be overlooked, regardless of the length of time that embryos remain in cryostorage.

There are no comments yet on this publication. Be the first to share your thoughts.