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Flow-injection determination of proteins based on the lowry spectrophotometric method

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
217
Identifiers
DOI: 10.1016/s0003-2670(00)80418-3

Abstract

Abstract The flow-injection system is based on the use of Folin-Ciocalteus reagent, with 1,4-dithio-D,L-threitol to accelerate the formation of the coloured complex. The calibration graph for bovine serum albumin is linear between 0.01 and 0.1 mg ml −1; the detection limit is 0.005 mg ml −1 albumin and up to 0.5 mg ml −1 can be determined. The injection frequency is 20 h −1. The results obtained by the flow-injection method and by the manual Lowry procedure show excellent correlation. Egg lysozyme, alkaline phosphatase and β-lactoglobulin are also tested.

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