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Dietary flavonoid quercetin stimulates vasorelaxation in aortic vessels

Authors
Journal
Free Radical Biology and Medicine
0891-5849
Publisher
Elsevier
Publication Date
Volume
49
Issue
3
Identifiers
DOI: 10.1016/j.freeradbiomed.2010.04.022
Keywords
  • Nitric Oxide Synthase
  • Endothelium
  • Cardioprotection
  • Enos
  • Heart
  • Catalase
  • Endothelium-Derived Hyperpolarizing Factor
  • Phosphorylation
  • Aortic Ring Segments
  • H2O2
  • Fura-2
  • Mircofluorometric Ca2+Imaging
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Abstract Considerable epidemiological evidence indicates that dietary consumption of moderate levels of polyphenols decreases both the incidence of cardiovascular disease and the mortality associated with myocardial infarction. Molecular mechanisms of this cardiovascular protection remain uncertain but can involve changes in rates of nitric oxide (NO) generation by endothelial nitric oxide synthase (eNOS). We examined the vascular responses to quercetin using a combination of biochemical and vessel function criteria. Quercetin treatment for 30 min enhanced relaxation of rat aortic ring segments. Moreover, the addition of L-NAME (100 μM) or charybdotoxin (ChTx) blocked quercetin-mediated vasorelaxation thus demonstrating the effect was partially dependent on NOS and endothelium-derived hyperpolarizing factor (EDHF). Additionally, bovine aortic endothelial cells (BAEC) treated with quercetin showed a rapid increase of intracellular Ca 2+ concentrations as well as a dose- and time-dependent stimulation of eNOS phosphorylation with a concomitant increase in NO production. These results demonstrate that quercetin-mediated stimulation of eNOS phosphorylation increases NO bioavailability in endothelial cells and can thus play a role in the vascular protective effects associated with improved endothelial cell function.

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