Affordable Access

Publisher Website

Isolation and Characterization of the Gene Encoding Rat Glucose-Dependent Insulinotropic Peptide

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
193
Issue
1
Identifiers
DOI: 10.1006/bbrc.1993.1607

Abstract

Abstract The rat glucose-dependent insulinotropic peptide (GIP) gene has been isolated and characterized. The gene spans approximately 8.2 kilobase pairs (kb) and the GIP mRNA (0.8 kb) is encoded by six exons. The 42 amino acid hormone is encoded by exons 3 and 4. The exon-intron organization of the rat GIP gene revealed that the splice acceptor site for intron 2 is 24 nucleotides downstream compared to the comparable splice acceptor site in the human gene. This intron sliding results in an 8 amino acid deletion in the amino terminal extension of the prepropeptide. Primer extention analysis and RNase protection assay demonstrated the existence of multiple closely spaced sites for transcriptional initiation. Both the 5′-flanking region and intron 1 contain TATA and CCAAT boxes consistent with initiation of gene transcription, although a TATA box in intron 1 is functionally inactive in adult rats in spite of its reasonable location.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Isolation and characterization of the gene encodin...

on Biochemical and Biophysical Re... May 28, 1993

Glucose-dependent insulinotropic peptide (GIP) gen...

on Molecular and Cellular Endocri... Jan 01, 1995

Glucose-dependent insulinotropic peptide (GIP) gen...

on Molecular and Cellular Endocri... Nov 30, 1995

Dietary regulation of glucose-dependent insulinotr...

on Comparative biochemistry and p... February 1995
More articles like this..