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Synthesis, characterisation and high-performance liquid chromatography of C6C16dicarboxylyl-mono-coenzyme A and -mono-carnitine esters

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
570
Issue
2
Identifiers
DOI: 10.1016/0378-4347(91)80529-l
Disciplines
  • Biology

Abstract

Abstract The synthesis and purification of the mono-coenzyme A and mono-carnitine esters of the homologous series of straight-chain even-numbered dicarboxylic acids (C 6C 16) is described. The corresponding 3-hydroxyacyl- and 2-enoyl-CoA esters were prepared enzymatically. A reversed-phase high-performance liquid chromatographic (HPLC) system for the analysis of the intact CoA esters is described and their chromatographic behaviour documented. Reversed-phase HPLC systems for the analysis of the 4-bromophenacyl derivatives of the dicarboxylyl-mono-carnitines and the 4-nitrobenzyl derivatives of the free acids are also described. Some preliminary studies of the metabolism of [U- 14C]hexadecanedionoyl-mono-CoA by rat liver peroxisomes and rat skeletal muscle mitochondria are describedillustrating the application of these methods.

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