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Expression of C-terminal truncated and full-lengthBabesia bigeminarhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay

Authors
Journal
Veterinary Parasitology
0304-4017
Publisher
Elsevier
Publication Date
Volume
137
Identifiers
DOI: 10.1016/j.vetpar.2005.12.025
Keywords
  • Babesia Bigemina
  • Rhoptry-Associated Protein 1
  • Recombinant Antigen
  • Enzyme-Linked Immunosorbent Assay
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Recombinant antigen-based enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of Babesia bigemina infection by using a full-length B. bigemina rhoptry-associated protein 1 (rRAP-1) and the truncated C-terminal RAP-1 (rRAP-1/CT). While the rRAP-1 showed cross reactivity between B. bigemina- and Babesia bovis-infected bovine sera, the rRAP-1/CT was highly specific to B. bigemina-infected bovine sera and proved useful in the detection of sequential sera collected from an experimentally infected cow during the acute and latent infection. The high yield of soluble rRAP-1/CT and its diagnostic specificity demonstrate its potential in the diagnosis of B. bigemina infection. Its usefulness for epidemiological investigation is currently being evaluated.

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