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The role of adenylate cyclase in relaxation of brain arteries: studies with forskolin

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
457
Issue
2
Identifiers
DOI: 10.1016/0006-8993(88)90691-9
Keywords
  • Cerebral Artery
  • Forskolin
  • Vasoactive Intestinal Polypeptide (Vip)
  • Adenylate Cyclase
  • Adenosine Monophosphate
  • Cyclic

Abstract

Abstract The natural product, forskolin, which stimulates adenylate cyclase by a direct, non-receptor-mediated mechanism, was studied for its effect on the tension of isolated brain arteries and adenylate cyclase activity of cerebral arteries. Helical strips of bovine and porcine basilar arteries and bovine middle cerebral arteries, which had been precontracted with prostaglandin F 2α (PGF 2α) or KCl, relaxed potently to administration of forskolin with ED 50 values, ranging from 22 to 69 nM. Incubation of forskolin with a broken cell preparation of bovine cerebral arteries resulted in an efficacious stimulation of adenylate cyclase, approximating 5 times basal activity at a forskolin concentration of 1 μM. The metal salts nickel chloride and manganese chloride decreased the potency of vasorelaxation by vasoactive intestinal peptide (VIP), which stimulates adenylate cyclase via the VIP receptor. In contrast, nickel chloride had little effect on vasorelaxation by forskolin. The endogenous nucleoside, adenosine, which acts via the adenosine receptor and adenylate cyclase, relaxed bovine basilar and middle cerebral arteries with ED 50 values ranging from 0.26 to 0.94 μM. The data presented support a role for adenylate cyclase in mediating vasodilation of brain blood vessels.

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