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Determination of trace enantiomeric impurities in chiral compounds by capillary electrophoresis with uncoated, cationic, anionic and neutral surface modified capillaries

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
817
Identifiers
DOI: 10.1016/s0021-9673(98)00245-3
Keywords
  • Propranolol
  • Warfarin
  • Tryptophan

Abstract

Abstract The usefulness of commercially available polyamine, sulphonic acid and neutral coated capillaries is demonstrated for the resolution of chiral drugs. The methods, using cyclodextrins and their derivatives are developed from conventional fused-silica capillary procedures without any changes to the procedures. Different coatings can be used to adjust the order of elution of enantiomers when one of the enantiomers is present as a trace level impurity. Limit of quantitation levels of <0.1% (m/m) have been illustrated for d- or l-tryptophan in the presence of the other enantiomer with acceptable levels of reproducibility. Similar levels have been detected for warfarin and propranolol, using a polyamine-coated capillary. The coated capillaries were stable to long-term changes in the electrolyte and its pH value and the methods were interchangeable with capillaries obtained from different batches.

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