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The production of high yields of infectious vesicular stomatitis virus inA. albopictuscells and comparisons with replication in BHK-21 cells

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
107
Issue
2
Identifiers
DOI: 10.1016/0042-6822(80)90317-7
Disciplines
  • Biology

Abstract

Abstract Yields of vesicular stomatitis virus (VSV) ranging between 6 × 10 8 and 3 × 10 9 PFU/ml were obtained from a clonally derived line (LT C-7) of A. albopictus cells which has been adapted to Eagle's medium. Both with respect to specific infectivity and PFUIHA ratio, VSV grown in A. albopictus cells (VSV A. albo ) could not be distinguished from virus grown in BHK cells. VSV A. albo however, contained less G and L protein (relative to N) than did VSV BHK. Although relative to the other viral proteins there was also less G protein in lysates of infected mosquito cells than in BHK cells, relative to the other viral messenger RNAs, the G protein mRNA was more abundant in A. albopictus cells than in BHK cells.

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