Affordable Access

Publisher Website

Identification of three CCp genes in Babesia divergens: Novel markers for sexual stages parasites

Authors
Journal
Molecular and Biochemical Parasitology
0166-6851
Publisher
Elsevier
Volume
174
Issue
1
Identifiers
DOI: 10.1016/j.molbiopara.2010.06.011
Keywords
  • Babesia Divergens
  • Plasmodium
  • Gametocytes
  • Sexual Stages
  • Ccp Family
Disciplines
  • Biology

Abstract

Abstract Babesia divergens, a tick-borne protozoan parasite of red blood cells, is the main agent of bovine and human babesiosis in Europe. Very few data are available concerning its life cycle and sexual reproduction inside the tick vector, Ixodes ricinus. The aim of this study was to define some markers of the B.divergens sexual stage. An in silico post-genomic approach was used to analyze genomic, transcriptomic and proteomic data and to select specific sexual stage proteins of the related apicomplexan genus Plasmodium. Three proteins, based on sequence identity between the available genomes of Plasmodium and Babesia spp., were chosen, as members of a highly conserved and apicomplexan sexual stages specific protein family (CCp) potentially involved in adhesive functions. Degenerate primers were used to amplify and clone three B.divergens orthologs (bdccp1, bdccp2, and bdccp3) corresponding to newly identified genes in this parasite. The opportunities offered by such markers to study parasite development in its vector are discussed.

There are no comments yet on this publication. Be the first to share your thoughts.