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A Multiplex PCR Assay for the Detection of Chlamydia, Gonorrhea, and Trichomonas Using Melt Curve Analysis

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  • Mathematics
  • Medicine


An estimated 448 million new cases of curable sexually transmitted infections (STIs) occur every year. Some of these STIs exist without symptoms and, if untreated, can have devastating consequences on reproduction, maternal and newborn health, as well as increase the likelihood of acquisition and transmission of HIV. Traditional methods for diagnosing STIs are laborious, often not very sensitive, and have a long turnaround time. Here, we describe the development of a novel, rapid and affordable diagnostic assay for the simultaneous detection of Trichomonas vaginalis (TV), Neisseria gonorrhea (NG), and Chlamydia trachomatis (CT).

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