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Differential association of the different brain microtubule proteins in different in vitro assembly conditions

Authors
Journal
Biochimica et Biophysica Acta (BBA) - General Subjects
0304-4165
Publisher
Elsevier
Publication Date
Volume
838
Issue
1
Identifiers
DOI: 10.1016/0304-4165(85)90246-6
Keywords
  • Microtubule Assembly
  • Protein Association
  • (Brain)
Disciplines
  • Biology

Abstract

Abstract Microtubule protein was assembled in vitro for different time periods and at different protein concentrations, near and far from the critical concentration needed for assembly. When the isolated polymers were characterized by electrophoresis, a higher association of high-molecular-weight microtubule-associated proteins, particularly of the larger microtubule-associated proteins, was found in the polymers assembled close to the critical concentration, while tau factor polypeptides are predominantly present in those polymers assembled at higher protein concentration. Also, a higher proportion of high-molecular-weight microtubule-associated proteins MAP 1 and MAP 2 was observed in microtubules assembled for short time periods, compared with those obtained after the monomer-polymer equilibrium was reached. When the assembled protein was further characterized by isoelectric focusing, no differences were found in the proportion of the different isotubulins present in the polymers assembled under different conditions tested.

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