Affordable Access

Publisher Website

Changes inN-acetyl-β-D-glucosaminidase binding system in rat liver during growth

Authors
Journal
Mechanisms of Ageing and Development
0047-6374
Publisher
Elsevier
Publication Date
Volume
86
Issue
2
Identifiers
DOI: 10.1016/0047-6374(95)01678-3
Keywords
  • N-Acetyl-β-D-Glucosaminidase
  • Receptors
  • Lysosomal Enzymes
  • Liver
Disciplines
  • Biology

Abstract

Abstract In this paper, we characterize the binding of N-acetyl-β-D-glucosaminidase to membranes of rat liver during development. Cation-independent phosphomannosyl receptors were shown to be involved in the recognition of the enzyme at the following ages: 18 days of fetal life, 10-, 30- and 90-day-old rats. We remark here two important differences in the binding of the fetal enzyme when compared with that in 90-day-old rats; (a) in addition to an optimal binding at pH 7.0, the fetal enzyme showed another peak of binding at acidic pH (5.0), and (b) the binding of the fetal enzyme was also inhibited by galactose-6-phosphate. This inhibition was higher than that of mannose-6-phosphate at the acidic pH. We concluded that the lysosomal enzymes and their receptors undergo important changes around birth.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Changes in N-acetyl-beta-D-glucosaminidase binding...

on Mechanisms of Ageing and Devel... Feb 24, 1996

Immunohistochemical localization of transforming g...

on Molecular and Cellular Endocri... Jan 01, 1992

Changes inN-acetyl-β-glucosaminidase activity in h...

on Biochimica et Biophysica Acta... Jan 01, 1968
More articles like this..