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Quantitative immunoelectrophoresis of serum proteins

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
10
Issue
2
Identifiers
DOI: 10.1016/0009-8981(64)90155-x
Disciplines
  • Biology

Abstract

Abstract A method of quantitative immunoelectrophoresis is described. Immunoelectrophoretic fractions of serum proteins are determined by single gradient diffusion. Antiserum diffusion gradient is eliminated by either incorporating antiserum in the agar gel before electrophoresis, or by applying it in uniform layer on the agar gel surface after electrophoresis. Transverse diameters of the precipitation patterns of standard protein solutions of different concentration are plotted to obtain a calibration curve from which the concentration of the unknown sample is read.

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