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Characteristics of the glucose oxidase at different surfaces

Authors
Journal
Bioelectrochemistry and Bioenergetics
0302-4598
Publisher
Elsevier
Publication Date
Volume
42
Issue
1
Identifiers
DOI: 10.1016/s0302-4598(96)05140-9
Keywords
  • Glucose Oxidase
  • Thiol
  • Self-Assembled-Monolayers
  • Immobilization
Disciplines
  • Chemistry

Abstract

Abstract By adsorption or chemical bonding, glucose oxidase (GOD) molecules are immobilized to different surfaces, including bare Pt and Au, alkanethiols self-assembled monolayers, and ω-carboxylic acid thiols self-assembled monolayers. Except Au and reduced Pt surfaces, GOD can be immobilized on all the surfaces tested. The most durable immobilization is achieved by covalent bonding GOD to carboxylic terminated SAM. In most cases the immobilized GOD retains its native enzymatic activity. A chain length dependence of the apparent Michealis constant is found for the GOD adsorbed at carboxylic group terminated SAM and the possible reasons are discussed.

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