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Latent transforming growth factor β-binding protein-3 and fibulin-1C interact with the extracellular domain of the heparin-binding EGF-like growth factor precursor

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Publisher
BioMed Central
Publication Date
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PMC
Keywords
  • Research Article
Disciplines
  • Biology

Abstract

1471-2121-3-2.fm ral BioMed CentBMC Cell Biology BMC Cell Biology 2002, 3Research article Latent transforming growth factor β-binding protein-3 and fibulin- 1C interact with the extracellular domain of the heparin-binding EGF-like growth factor precursor Joanna S Brooke*1, Jeong-Heon Cha2 and Leon Eidels2 Address: 1Present adress: Dept of Biological Science, Depaul University, Chicago, Il 60614, USA and 2Department of Microbiology, The University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, Texas 75390-9048, USA E-mail: Joanna S Brooke* - [email protected]; Jeong-Heon Cha - [email protected]; Leon Eidels - [email protected] *Corresponding author Abstract Background: The membrane-bound cell-surface precursor and soluble forms of heparin-binding epidermal growth factor-like growth factor (HB-EGF) contribute to many cellular developmental processes. The widespread occurrence of HB-EGF in cell and tissue types has led to observations of its role in such cellular and tissue events as tumor formation, cell migration, extracellular matrix formation, wound healing, and cell adherence. Several studies have reported the involvement of such extracellular matrix proteins as latent transforming growth factor β-binding protein, TGF-β, and fibulin-1 in some of these processes. To determine whether HB-EGF interacts with extracellular matrix proteins we used the extracellular domain of proHB-EGF in a yeast two-hybrid system to screen a monkey kidney cDNA library. cDNA clones containing nucleotide sequences encoding domains of two proteins were obtained and their derived amino acid sequences were evaluated. Results: From � 3 × 106 screened monkey cDNA clones, cDNA clones were recovered that contained nucleotide sequences encoding domains of the monkey latent transforming growth factor-β binding protein-3 (MkLTBP-3) and fibulin-1C protein. The amino acid sequence derived from the MkLTBP-3 gene shared 98.6% identity with human LTBP-3 and 86.7% ide

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