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Production of cytidine 5′-diphosphorylcholine with high utilization of ATP by whole cells ofSaccharomycescerevisiae

Authors
Journal
Bioresource Technology
0960-8524
Publisher
Elsevier
Publication Date
Volume
101
Issue
22
Identifiers
DOI: 10.1016/j.biortech.2010.06.006
Keywords
  • Cytidine 5′-Diphosphorylcholine
  • Atp Regeneration
  • Choline Chloride
  • Saccharomyces Cerevisiae
Disciplines
  • Design

Abstract

Abstract Cytidine 5′-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomyces cerevisiae As 2.398. Out of eight variables, KH 2PO 4, glycerol and (NH 4) 2SO 4 were considered to be the most significant factors by response surface methodology including a Plackett–Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13 g/L KH 2PO 4, 12.35 g/L glycerol and 0.49 g/L (NH 4) 2SO 4, respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.

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