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Ubiquitous and Endoplasmic Reticulum–Located Lysophosphatidyl Acyltransferase, LPAT2, Is Essential for Female but Not Male Gametophyte Development in Arabidopsis

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Publisher
American Society of Plant Biologists
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PMC
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  • Research Articles

Abstract

Ubiquitous and Endoplasmic Reticulum–Located Lysophosphatidyl Acyltransferase, LPAT2, Is Essential for Female but Not Male Gametophyte Development in Arabidopsis by Hyun Uk Kim, Yubing Li, and Anthony H.C. Huang Plantcell Volume 17(4):1073-1089 April 1, 2005 ©2005 by American Society of Plant Biologists An Unrooted Phylogenetic Tree of Five Putative LPATs of Arabidopsis and Related LPATs of Other Plant Species Constructed on the Basis of Their Predicted Amino Acid Sequences.The LPATs of E. coli and yeast are included for comparison. Kim H et al. Plantcell 2005;17:1073-1089 ©2005 by American Society of Plant Biologists Structures of Four Putative Arabidopsis LPAT Genes Encoding Cytoplasmic LPATs and Their Predicted Amino Acid Sequences.Top panel: the exons (boxes) and introns (lines) of the four genes. Kim H et al. Plantcell 2005;17:1073-1089 ©2005 by American Society of Plant Biologists Expression of LPAT2 and LPAT3 in the E. coli Strain JC201, a Temperature-Sensitive Mutant Defective in LPAT.(A) Bacteria transformed with pQE, pQE-LPAT2, or pQE-LPAT3 were grown at 30° C or 42° C for 18 h and photographed. pQE-LPAT2 (shown) but not pQE-LPAT... Kim H et al. Plantcell 2005;17:1073-1089 ©2005 by American Society of Plant Biologists Expression of LPAT2-5 in Saccharomyces cerevisiae.(A) Yeast transformed with pYES or pYES-LPAT2-5 were analyzed for the presence of LPAT2-5 in DNA (cells grown in glucose) and LPAT2-5 transcripts in RNA (cells grown in glucose [Glu] and then transferred to ... Kim H et al. Plantcell 2005;17:1073-1089 ©2005 by American Society of Plant Biologists RT-PCR Analyses of the Transcripts of LPAT2-5 in Various Organs.The organs included siliques (Si), unopened flowers (F), rosette leaves (RL), stems (St), roots (R), and early (E) and late (L) maturing embryos and seedlings. Kim H et al. Plantcell 2005;17:1073-1089 ©2005 by American Society of Plant Biologists Transcripts of the Four Putative Genes in Different Organs with Use of the MPSS Database.Information was obt

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