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Characterization ofPseudoalteromonas citreaandP. nigrifaciensIsolated from Different Ecological Habitats Based on REP-PCR Genomic Fingerprints

Authors
Journal
Systematic and Applied Microbiology
0723-2020
Publisher
Elsevier
Publication Date
Volume
25
Issue
2
Identifiers
DOI: 10.1078/0723-2020-00103
Keywords
  • Pseudoalteromonas
  • Biogeography
  • Rep
  • Eric
  • Box-Pcr
Disciplines
  • Biology
  • Earth Science
  • Ecology
  • Geography

Abstract

Summary DNA primers corresponding to conserved repetitive interspersed genomic motifs and PCR were used to show that REP, ERIC and BOX-like DNA sequences are present in marine, oxidative, Gram-negative Pseudoalteromonas strains. REP, ERIC and BOX-PCR were used for rapid molecular characterization of both the type species of the genus and environmental strains isolated from samples collected in different geographical areas. PCR-generated genomic fingerprint patterns were found to be both complex and strain specific. Analysis of the genotypic structure of phenotypically diverse P. citrea revealed a geographic clustering of Far Eastern brown-pigmented, agar-digesting strains of this species. Marine isolates of P. nigrifaciens with 67–70% DNA relatedness generated genomic patterns different from those of the type strain and formed a separate cluster. It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion.

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